Atrial natriuretic factor and cyclic guanosine 3',5'-monophosphate in vascular smooth muscle

Makito Sato, Keishi Abe, Kazuhisa Takeuchi, Minoru Yasujima, Ken Omata, Masao Hiwatari, Yutaka Kasai, Masaya Tanno, Masahiro Kohzuki, Kei Kudo, Kaoru Yoshinaga, Tadashi Inagami

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Abstract

To elucidate the molecular mechanism of the vascular action of atrial natriuretic factor (ANF), we investigated the effects of synthetic ANF and sodium nitroprusside on the levels of intracellular cyclic nucleotides and prostacyclin (measured as its stable metabolite 6-keto-prostaglandin F,J in cultured vascular smooth muscle cells from rat mesenteric artery and, in some experiments, from rat renal artery. Both ANF and sodium nitroprusside increased intracellular cyclic guanosine 3',5'- monophosphate (cGMP) levels in a dose-dependent manner but did not affect cyclic adenosine 3',5'- monophosphate levels or 6-keto-prostaglandin F synthesis. The stimulatory effects of ANF and sodium nitroprusside on cGMP levels were additive. Neither the deprivation of extracellular Ca2+ nor calcium entry blockers affected ANF-stimulated cGMP levels. Preincubation of ANF or sodium nitroprusside with kallikrein attenuated only the effect of ANF on cGMP levels. The effect of kallikrein was abolished by serine protease inhibitors. In contrast, the oxidant methylene blue inhibited the effect of sodium nitroprusside on cGMP levels, but not that of ANF. The stimulatory effect of ANF on cGMP levels was greater in cells from renal artery than in those from mesenteric artery. These results in cultured vascular smooth muscle cells further support the hypothesis that cGMP mediates the vasorelaxant action of ANF.

Original languageEnglish
Article number00007
Pages (from-to)762-771
Number of pages10
JournalHypertension
Volume8
Issue number9
DOIs
Publication statusPublished - 1986 Sep

Keywords

  • Atrial natriuretic factor
  • Cyclic guanosine 3',5'-monophosphate
  • Kallikrein
  • Methylene blue
  • Sodium nitroprusside
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Internal Medicine

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  • Cite this

    Sato, M., Abe, K., Takeuchi, K., Yasujima, M., Omata, K., Hiwatari, M., Kasai, Y., Tanno, M., Kohzuki, M., Kudo, K., Yoshinaga, K., & Inagami, T. (1986). Atrial natriuretic factor and cyclic guanosine 3',5'-monophosphate in vascular smooth muscle. Hypertension, 8(9), 762-771. [00007]. https://doi.org/10.1161/01.HYP.8.9.762