AtPex14p maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes

Makoto Hayashi, Kazumasa Nito, Kanako Toriyama-Kato, Maki Kondo, Tomoyuki Yamaya, Mikio Nishimura

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

We previously isolated an Arabidopsis peroxisome-deficient ped2 mutant by its resistance to 2,4-dichloro-phenoxybutyric acid. Here, we describe the isolation of a gene responsible for this deficiency, called the PED2 gene, by positional cloning and confirmed its identity by complementation analysis. The amino acid sequence of the predicted protein product is similar to that of human Pex14p, which is a key component of the peroxisomal protein import machinery. Therefore, we decided to call it AtPex14p. Analyses of the ped2 mutant revealed that AtPexl4p controls intracellular transport of both peroxisome targeting signal (PTS)1- and PTS2-containing proteins into three different types of peroxisomes, namely glyoxysomes, leaf peroxisomes and unspecialized peroxisomes. Mutation in the PED2 gene results in reduction of enzymes in all of these functionally differentiated peroxisomes. The reduction in these enzymes induces pleiotropic defects, such as fatty acid degradation, photorespiration and the morphology of peroxisomes. These data suggest that the AtPexl4p has a common role in maintaining physiological functions of each of these three kinds of plant peroxisomes by determining peroxisomal protein targeting.

Original languageEnglish
Pages (from-to)5701-5710
Number of pages10
JournalEMBO Journal
Volume19
Issue number21
DOIs
Publication statusPublished - 2000 Nov 1

Keywords

  • Peroxisome
  • Pex14
  • Photorespiration
  • Protein targeting
  • β-Oxidation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint

Dive into the research topics of 'AtPex14p maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes'. Together they form a unique fingerprint.

Cite this