Atomic force microscopy observation of membrane proteins suspended over carbon nanotube network

Koji Sumitomo, Youichi Shinozaki, Daisuke Takagi, Hiroshi Nakashima, Yoshihiro Kobayashi, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Atomic force microscopy (AFM) imaging of membrane proteins suspended over a nanostructure in liquid is a promising way to understand the structure and function of working proteins, although the membrane deformation that occurs during scanning makes it difficult to obtain a high resolution image. This study proposes an artificial cell system for the AFM observation of functional membrane proteins that consists of a sub-micron well on Si, a biological membrane, and a carbon nanotube (CNT) network. We successfully observed molecular-scale images of a purple membrane suspended over sub-micron well patterns. By using a CNT network to hold the suspended membrane, we suppressed the membrane deformation caused by the "imaging force". The CNT network takes the place of a cytoskeleton in supporting the cell membrane suspended over the well thus improving the spatial resolution of AFM measurement.

Original languageEnglish
Article number08JB18
JournalJapanese journal of applied physics
Volume48
Issue number8 PART 3
DOIs
Publication statusPublished - 2009 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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