Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity

Sara E. Hanbal, Keisuke Takashima, Shuhei Miyashita, Sugihiro Ando, Kumiko Ito, Mohsen M. Elsharkawy, Toshiro Kaneko, Hideki Takahashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Low-temperature atmospheric-pressure air plasma is a source of charged and neutral gas species. In this study, N-carrying tobacco plants were inoculated with plasma irradiated and non-irradiated tobacco mosaic virus (TMV) solution, resulting in necrotic local lesions on non-irradiated, but not on irradiated, TMV-inoculated leaves. Virus particles were disrupted by plasma irradiation in an exposure-dependent manner, but the viral coat protein subunit was not. TMV RNA was also fragmented in a time-dependent manner. These results indicate that plasma irradiation of TMV can collapse viral particles to the subunit level, degrading TMV RNA and thereby leading to a loss of infectivity.

Original languageEnglish
Pages (from-to)2835-2840
Number of pages6
JournalArchives of virology
Volume163
Issue number10
DOIs
Publication statusPublished - 2018 Oct 1

ASJC Scopus subject areas

  • Virology

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