Asymmetrically functional surface properties on biocompatible phospholipid polymer membrane for bioartificial kidney

Hideto Ueda, Junji Watanabe, Tomohiro Konno, Madoka Takai, Akira Saito, Kazuhiko Ishihara

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

To obtain a bioartificial kidney composed of a porous polymer membrane and renal cells, a polysulfone (PSf) membrane (PSM) blended with 2-methacryloyloxy-ethyl phosphorylcholine (MPC) polymer was prepared. The PSM flat membrane with a porous structure could be prepared from the polymer blend containing 1 wt % of the MPC polymer in PSf by the phase inversion technique in a dry-wet process. Asymmetrical surface properties were observed on both sides of the membrane surfaces. That is, the sponge layer formed at the substrate-contacting surface of the membrane had 10-20 μm pores, but the pores in the micrometer range could not be observed for a skin layer formed at the air-contacting surface of the membrane. At the sponge layer surface, the MPC unit composition was 7 times larger than that at the skin layer surface. The amount of proteins adsorbed on the surface corresponded to the MPC unit composition. On the skin layer, a small amount of adsorbed proteins and platelet adhesion could be suppressed compared with those on the sponge layer. However, the skin layer had a moderate protein adsorption, so it showed a sufficient cytocompatibility to enable renal tubule epithelial cells to adhere and proliferate in the membrane. Thus, it functioned well as a renal tubule. Therefore, because of both its hemocompatibility and cytocompatibility, we could conclude that the PSM membrane is useful for as a renal tubule device for a bioartificial kidney.

Original languageEnglish
Pages (from-to)19-27
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume77
Issue number1
DOIs
Publication statusPublished - 2006 Apr 1
Externally publishedYes

Keywords

  • Artificial kidney
  • Cytocompatibility
  • Hemocompatibility
  • Phospholipid polymer
  • Polysulfone

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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