Astrocytes in Atp1a2-deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression

Hiroki Sugimoto, Masaaki Sato, Junichi Nakai, Kiyoshi Kawakami

Research output: Contribution to journalArticle

Abstract

The ATP1A2 coding α2 subunit of Na,K-ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2tmCKwk/+) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and headache. However, astrocytic properties during CSD have not been examined in FHM2 model mice. Using Atp1a2tmCKwk/+ crossed with transgenic mice expressing G-CaMP7 in cortical neurons and astrocytes (Atp1a2+/−), we analyzed the changes in Ca2+ concentrations during CSD. The propagation speed of Ca2+ waves and the percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− were higher than those in wild-type mice. Increased percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− may contribute to FHM2 pathophysiology.

Original languageEnglish
Pages (from-to)1031-1043
Number of pages13
JournalFEBS Open Bio
Volume10
Issue number6
DOIs
Publication statusPublished - 2020 Jun 1

Keywords

  • Atp1a2
  • CSD
  • FHM2
  • G-CaMP7
  • astrocyte
  • calcium imaging
  • migraine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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