Association between up-regulation of stress-responsive genes and hypomethylation of genomic DNA in tobacco plants

Y. Wada, K. Miyamoto, T. Kusano, H. Sano

    Research output: Contribution to journalArticlepeer-review

    140 Citations (Scopus)

    Abstract

    Transcripts that specifically accumulate in transgenic tobacco plants expressing an anti-sense construct for a tobacco type I DNA methyltransferase, NtMET1, were screened by the differential display method. Of the 31 genes identified, 16 encoded proteins with known functions; ten of these were related to biotic and abiotic stress responses, and the other six to cellular functions. In order to examine whether expression of these genes is correlated with DNA methylation status under natural stress conditions, a pathogen-responsive gene (NtAlix1) was selected as representative, and assayed for transcript induction and genomic methylation in tobacco plants infected with tobacco mosaic virus (TMV). In inoculated leaves of wild-type plants, NtAlix1 transcripts began to accumulate 12 h after the onset of the hypersensitive response (HR), and levels remained high for up to 24 h. Changes in the methylation status at the locus became obvious 24 h later, as detected by digestion of genomic DNA with a methylation-sensitive restriction enzyme. The results suggest that the level of DNA methylation may change in response to external stresses, and that this is closely related to the activation of stress-responsive genes.

    Original languageEnglish
    Pages (from-to)658-666
    Number of pages9
    JournalMolecular Genetics and Genomics
    Volume271
    Issue number6
    DOIs
    Publication statusPublished - 2004 Jul

    Keywords

    • 5-Methylcytosine
    • DNA methyltransferase
    • Hypersensitive response
    • Nicotiana tabacum
    • Tobacco mosaic virus

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Fingerprint

    Dive into the research topics of 'Association between up-regulation of stress-responsive genes and hypomethylation of genomic DNA in tobacco plants'. Together they form a unique fingerprint.

    Cite this