Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET

Maolin Lu, Xiaochu Ma, Luis R. Castillo-Menendez, Jason Gorman, Nirmin Alsahafi, Utz Ermel, Daniel S. Terry, Michael Chambers, Dongjun Peng, Baoshan Zhang, Tongqing Zhou, Nick Reichard, Kevin Wang, Jonathan R. Grover, Brennan P. Carman, Matthew R. Gardner, Ivana Nikić-Spiegel, Akihiro Sugawara, James Arthos, Edward A. LemkeAmos B. Smith, Michael Farzan, Cameron Abrams, James B. Munro, Adrian B. McDermott, Andrés Finzi, Peter D. Kwong, Scott C. Blanchard, Joseph G. Sodroski, Walther Mothes

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)


The HIV-1 envelope glycoprotein (Env) trimer mediates cell entry and is conformationally dynamic1–8. Imaging by single-molecule fluorescence resonance energy transfer (smFRET) has revealed that, on the surface of intact virions, mature pre-fusion Env transitions from a pre-triggered conformation (state 1) through a default intermediate conformation (state 2) to a conformation in which it is bound to three CD4 receptor molecules (state 3)8–10. It is currently unclear how these states relate to known structures. Breakthroughs in the structural characterization of the HIV-1 Env trimer have previously been achieved by generating soluble and proteolytically cleaved trimers of gp140 Env that are stabilized by a disulfide bond, an isoleucine-to-proline substitution at residue 559 and a truncation at residue 664 (SOSIP.664 trimers)5,11–18. Cryo-electron microscopy studies have been performed with C-terminally truncated Env of the HIV-1JR-FL strain in complex with the antibody PGT15119. Both approaches have revealed similar structures for Env. Although these structures have been presumed to represent the pre-triggered state 1 of HIV-1 Env, this hypothesis has never directly been tested. Here we use smFRET to compare the conformational states of Env trimers used for structural studies with native Env on intact virus. We find that the constructs upon which extant high-resolution structures are based predominantly occupy downstream conformations that represent states 2 and 3. Therefore, the structure of the pre-triggered state-1 conformation of viral Env that has been identified by smFRET and that is preferentially stabilized by many broadly neutralizing antibodies—and thus of interest for the design of immunogens—remains unknown.

Original languageEnglish
Pages (from-to)415-419
Number of pages5
Issue number7752
Publication statusPublished - 2019 Apr 18
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET'. Together they form a unique fingerprint.

Cite this