Assessment of utility of meiosis-associated promoters of lily for induction of germinal Ds transposition in transgenic rice

Ryouhei Morita, Yoko Hattori, Shuji Yokoi, Hisabumi Takase, Masayoshi Minami, Kazuyuki Hiratsuka, Kinya Toriyama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In order to suppress the somatic excision of the Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. The LIM10 promoter directed GUS expression specifically in anthers, with the LIM18 promoter doing the same in the anthers and somatic tissue. Both promoters induced independent germinal transposition with the frequency of approximately 1%. The LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.

Original languageEnglish
Pages (from-to)637-642
Number of pages6
JournalPlant and Cell Physiology
Volume44
Issue number6
DOIs
Publication statusPublished - 2003 Jun 1

Keywords

  • Ds transposition
  • Meiosis-associated promoter
  • Oryza sativa L.
  • Transposon Ac/Ds

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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