TY - JOUR
T1 - Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis
AU - Sato, Akiko
AU - Hiura, Hitoshi
AU - Okae, Hiroaki
AU - Miyauchi, Naoko
AU - Abe, Yukiko
AU - Utsunomiya, Takafumi
AU - Yaegashi, Nobuo
AU - Arima, Takahiro
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2011/1
Y1 - 2011/1
N2 - Objective: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. Design: Experimental prospective study. Setting: University research laboratory and private in vitro fertilization (IVF) clinic. Patient(s): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. Intervention(s): The ejaculated sperm samples after the routine semen analysis with patients' consent. Main Outcome Measure(s): Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). Result(s): A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). Conclusion(s): The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.
AB - Objective: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. Design: Experimental prospective study. Setting: University research laboratory and private in vitro fertilization (IVF) clinic. Patient(s): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. Intervention(s): The ejaculated sperm samples after the routine semen analysis with patients' consent. Main Outcome Measure(s): Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). Result(s): A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). Conclusion(s): The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.
KW - BPL
KW - Bisulfite PCR-Luminex methylation analysis
KW - DNA methylation
KW - genomic imprinting
KW - oligozoospermia
KW - sperm
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U2 - 10.1016/j.fertnstert.2010.06.076
DO - 10.1016/j.fertnstert.2010.06.076
M3 - Article
C2 - 20655520
AN - SCOPUS:78650414907
VL - 95
SP - 129-134.e4
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 1
ER -