Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis

Akiko Sato, Hitoshi Hiura, Hiroaki Okae, Naoko Miyauchi, Yukiko Abe, Takafumi Utsunomiya, Nobuo Yaegashi, Takahiro Arima

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Objective: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. Design: Experimental prospective study. Setting: University research laboratory and private in vitro fertilization (IVF) clinic. Patient(s): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. Intervention(s): The ejaculated sperm samples after the routine semen analysis with patients' consent. Main Outcome Measure(s): Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). Result(s): A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). Conclusion(s): The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.

Original languageEnglish
Pages (from-to)129-134.e4
JournalFertility and sterility
Volume95
Issue number1
DOIs
Publication statusPublished - 2011 Jan

Keywords

  • BPL
  • Bisulfite PCR-Luminex methylation analysis
  • DNA methylation
  • genomic imprinting
  • oligozoospermia
  • sperm

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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