Assembly of synthetic cellulose I

Jong H. Lee; R. Malcolm Brown; Shigenori Kuga; Shin Ichiro Shoda; Shiro Kobayashi

doi:10.1073/pnas.91.16.7425

Assembly of synthetic cellulose I

Jong H. Lee, R. Malcolm Brown, Shigenori Kuga, Shin Ichiro Shoda, Shiro Kobayashi

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

90 Citations (Scopus)

Abstract

Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of β-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph.

Original language	English
Pages (from-to)	7425-7429
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	16
DOIs	https://doi.org/10.1073/pnas.91.16.7425
Publication status	Published - 1994 Aug 2

Keywords

Trichoderma viride
cellulase
enzymatic polymerization
β-cellobiosyl fluoride

ASJC Scopus subject areas

General

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abstract = "Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of β-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph.",

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T1 - Assembly of synthetic cellulose I

AU - Lee, Jong H.

AU - Brown, R. Malcolm

AU - Kuga, Shigenori

AU - Shoda, Shin Ichiro

AU - Kobayashi, Shiro

PY - 1994/8/2

Y1 - 1994/8/2

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AB - Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of β-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph.

KW - Trichoderma viride

KW - cellulase

KW - enzymatic polymerization

KW - β-cellobiosyl fluoride

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Assembly of synthetic cellulose I

Abstract

Keywords

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