Assembly of synthetic cellulose I

Jong H. Lee, R. Malcolm Brown, Shigenori Kuga, Shin Ichiro Shoda, Shiro Kobayashi

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)


Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of β-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph.

Original languageEnglish
Pages (from-to)7425-7429
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number16
Publication statusPublished - 1994 Aug 2


  • Trichoderma viride
  • cellulase
  • enzymatic polymerization
  • β-cellobiosyl fluoride

ASJC Scopus subject areas

  • General

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