Assembly and connection of micropatterned single neurons for neuronal network formation

Shotaro Yoshida, Midori Kato-Negishi, Shoji Takeuchi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Engineering of neuronal network geometry by micropatterning technology is a key future technology for creating artificial brains on a chip. However, engineering of network geometry at the single-cell-level with functional morphology (axon/dendrite) and connectivity (synapses) is still challenging. Here, we describe a method for controlling the axon and dendrite morphology of single primary-cultured neurons and assembling a neural circuit using mobile microplates. The microplates enabled morphological control of neurons by their shapes and bringing their ends into contact caused the formation of physical connections. Functional synapse formation at the connection was indicated by immunostaining of synapse-related proteins and intracellular Ca2+ imaging of neural activity. We believe that the method will be useful in engineering neural circuits with selected neurons and defined morphology.

Original languageEnglish
Article number235
JournalMicromachines
Volume9
Issue number5
DOIs
Publication statusPublished - 2018 May 15

Keywords

  • Hippocampal neuron
  • Micromanipulation
  • Micropatterning
  • Neuronal network
  • Synapse

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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