Assays of PCB congeners and organochlorine insecticides with the transgenic Arabidopsis and tobacco plants carrying recombinant guinea pig AhR and GUS reporter genes

Keiko Gion, Hideyuki Inui, Hideaki Sasaki, Yasushi Utani, Susumu Kodama, Hideo Ohkawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of β-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g-1 PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.

Original languageEnglish
Pages (from-to)599-607
Number of pages9
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume47
Issue number7
DOIs
Publication statusPublished - 2012 Aug

Keywords

  • Polychlorinated biphenyl
  • guinea pig aryl hydrocarbon receptor
  • organochlorine insecticide
  • transgenic plants

ASJC Scopus subject areas

  • Food Science
  • Pollution

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