Assays for autophagy I: The cvt pathway and nonselective autophagy

Wei Pang Huang, Takahiro Shintani, Zhiping Xie

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Autophagy is a major intracellular degradation pathway. It is responsible for the bulk removal of obsolete or damaged cytoplasmic components, including both soluble proteins and membrane-bound organelles. As a fundamental function of eukaryotic cells, autophagy plays a key role in protecting the cells from stressful conditions. Budding yeast Saccharomyces cerevisiae has been the pioneering model system in autophagy-related research. In this chapter, we describe three basic assays of autophagy in S. cerevisiae: the Ape1 maturation assay, the GFP-Atg8 processing assay, and the Pho8Δ60 assay. These assays cover the selective cytoplasm to vacuole targeting (Cvt) pathway and starvation-induced nonselective autophagy.

Original languageEnglish
Pages (from-to)153-164
Number of pages12
JournalMethods in Molecular Biology
Volume1163
DOIs
Publication statusPublished - 2014 Jan 1

Keywords

  • Alkaline phosphatase
  • Ape1
  • Atg8
  • Autophagic flux
  • Autophagy
  • Cvt pathway
  • Pho8
  • Protocol
  • Yeast

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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