Assays for autophagy I: The cvt pathway and nonselective autophagy

Wei Pang Huang, Takahiro Shintani, Zhiping Xie

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Autophagy is a major intracellular degradation pathway. It is responsible for the bulk removal of obsolete or damaged cytoplasmic components, including both soluble proteins and membrane-bound organelles. As a fundamental function of eukaryotic cells, autophagy plays a key role in protecting the cells from stressful conditions. Budding yeast Saccharomyces cerevisiae has been the pioneering model system in autophagy-related research. In this chapter, we describe three basic assays of autophagy in S. cerevisiae: the Ape1 maturation assay, the GFP-Atg8 processing assay, and the Pho8Δ60 assay. These assays cover the selective cytoplasm to vacuole targeting (Cvt) pathway and starvation-induced nonselective autophagy.

    Original languageEnglish
    Pages (from-to)153-164
    Number of pages12
    JournalMethods in Molecular Biology
    Volume1163
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Alkaline phosphatase
    • Ape1
    • Atg8
    • Autophagic flux
    • Autophagy
    • Cvt pathway
    • Pho8
    • Protocol
    • Yeast

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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