Aspergillus oryzae atfB encodes a transcription factor required for stress tolerance in conidia

Kazutoshi Sakamoto, Toshi hide Arima, Kazuhiro Iwashita, Osamu Yamada, Katsuya Gomi, Osamu Akita

    Research output: Contribution to journalArticlepeer-review

    46 Citations (Scopus)


    Using an Aspergillus oryzae EST database, we identified a gene encoding a transcription factor (atfB), which is a member of the ATF/CREB family. Expression of atfB was barely detectable during vegetative growth, but was readily detected during conidiation in solid-state culture. Microarray analyses showed that expression of many other genes, including catalase (catA), were downregulated in an atfB-disruptant. The expression of most of these genes was upregulated in the wild-type strain during the conidiation phase in solid-state culture, and the expression pattern was similar to that of atfB itself. In the absence of stress, e.g. heat-shock or hydrogen peroxide, the conidial germination ratios for the ΔatfB strain and the wild-type strain were similar, but the stress tolerance of conidia carrying the ΔatfB deletion was less than that of the wild-type conidia. CRE-like DNA motifs, which are bound by ATF/CREB proteins, were found in the promoters of most of the downregulated genes in the ΔatfB strain. Thus, atfB appears to encode a transcription factor required for stress tolerance in conidia.

    Original languageEnglish
    Pages (from-to)922-932
    Number of pages11
    JournalFungal Genetics and Biology
    Issue number6
    Publication statusPublished - 2008 Jun


    • ATF/CREB
    • Aspergillus oryzae
    • Catalase
    • Oxidative stress
    • Stress tolerance
    • atfB
    • cDNA microarray
    • catA

    ASJC Scopus subject areas

    • Microbiology
    • Genetics


    Dive into the research topics of 'Aspergillus oryzae atfB encodes a transcription factor required for stress tolerance in conidia'. Together they form a unique fingerprint.

    Cite this