Aspergillus oryzae atfA controls conidial germination and stress tolerance

Kazutoshi Sakamoto, Kazuhiro Iwashita, Osamu Yamada, Ken Kobayashi, Akihiro Mizuno, Osamu Akita, Shigeaki Mikami, Hitoshi Shimoi, Katsuya Gomi

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42 Citations (Scopus)

Abstract

We compared atfA and atfB, the genes encoding the respective ATF/CREB-type transcription factors in Aspergillus oryzae. The germination ratio of ΔatfA conidia was low without any stress, unlike that of ΔatfB conidia. The ΔatfA conidia were more sensitive to oxidative stress than the ΔatfB conidia, which are also sensitive to oxidative stress. We compared the gene expressions of these strains by using a DNA microarray, GeneChip. Almost all the genes regulated by atfB were also regulated by atfA, but atfA also regulated many genes that were not regulated by atfB, including some genes putatively involved in oxidative stress resistance. The level of glutamate, the major amino acid in A. oryzae conidia, was significantly low only in the ΔatfA conidia, and the glycerol accumulation during germination was not observed only in the ΔatfA strain. We therefore concluded that atfA is involved in germination via carbon and nitrogen source metabolism. Crown

Original languageEnglish
Pages (from-to)887-897
Number of pages11
JournalFungal Genetics and Biology
Volume46
Issue number12
DOIs
Publication statusPublished - 2009 Dec 1

Keywords

  • ATF/CREB
  • Aspergillus oryzae
  • Catalase
  • GeneChip
  • Germination
  • Microarray analysis
  • Oxidative stress
  • atfA
  • atfB
  • catA

ASJC Scopus subject areas

  • Microbiology
  • Genetics

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    Sakamoto, K., Iwashita, K., Yamada, O., Kobayashi, K., Mizuno, A., Akita, O., Mikami, S., Shimoi, H., & Gomi, K. (2009). Aspergillus oryzae atfA controls conidial germination and stress tolerance. Fungal Genetics and Biology, 46(12), 887-897. https://doi.org/10.1016/j.fgb.2009.09.004