Ascites of severe acute pancreatitis in rats transcriptionally up- regulates expression of interleukin-6 and -8 in vascular endothelium and mononuclear leukocytes

Atsushi Masamune, Toru Shimosegawa, Motokazu Fujita, Akihiko Satoh, Masaru Koizumi, Takayoshi Toyota

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The molecular mechanisms that link acute pancreatitis and multiple organ failure remain unknown. We examined the effect of ascitic fluids prepared from rats with experimental necrotizing pancreatitis on the expression of interleukin (IL) -6 and IL-8 in human umbilical vein endothelial cells (HUVEC) and human monocytic THP-1 cells. Incubation of HUVEC or THP-1 cells with the ascitic fluids resulted in a concentration-dependent up-regulation of the cytokine expression with comparable mRNA induction. Electrophoretic mobility shift assay revealed that the ascitic fluids increased the nuclear factor-κB (NF-κ̄) and NF-IL6 binding activities. Intraperitoneal injection of ascitic fluids into healthy rats induced the activation of NF-κB in the infiltrating leukocytes in the lung. Our results suggested that ascitic fluids may play a role in the pathophysiology of severe acute pancreatitis through the activation of transcription factors and consequent cytokine productions in distant organs.

Original languageEnglish
Pages (from-to)429-437
Number of pages9
JournalDigestive Diseases and Sciences
Volume45
Issue number2
DOIs
Publication statusPublished - 2000 Mar 16

Keywords

  • Acute pancreatitis
  • Cytokine
  • Endothelial cells
  • Nuclear factor-κB
  • Transcription factor

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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