TY - JOUR
T1 - Aryl hydrocarbon receptor/dioxin receptor in human monocytes and macrophages
AU - Komura, K.
AU - Hayashi, S. I.
AU - Makino, I.
AU - Poellinger, L.
AU - Tanaka, H.
N1 - Funding Information:
We thank Drs. K. Okamoto, Y. Makino, F. Hirano, N. Yoshi-kawa, E. Fukawa, T. Miura for fruitful discussion and various support. This work is supported in part by a grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to H.T. and S.H.).
PY - 2001
Y1 - 2001
N2 - Aryl hydrocarbon receptor (AhR) belongs to the bHLH/PAS transcription factor family and is activated by various polycyclic or halogenated aromatic hydrocarbons, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3MC). In the present study, we showed that in U937 cells and human macrophages AhR, with its partner cofactor Arnt, is expressed and CYP1A1 mRNA expression is induced in the presence of AhR ligand 3MC. Moreover, we showed that AhR, associating with Arnt, binds to target DNA sequences and activates transcription. Since part of AhR is activated into DNA binding species in the absence of exogenous ligand and competitive AhR antagonist α-naphthoflavone inhibits this activation process with reducing CYP1A1 mRNA expression levels, the presence of endogenous ligand is indicated.
AB - Aryl hydrocarbon receptor (AhR) belongs to the bHLH/PAS transcription factor family and is activated by various polycyclic or halogenated aromatic hydrocarbons, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3MC). In the present study, we showed that in U937 cells and human macrophages AhR, with its partner cofactor Arnt, is expressed and CYP1A1 mRNA expression is induced in the presence of AhR ligand 3MC. Moreover, we showed that AhR, associating with Arnt, binds to target DNA sequences and activates transcription. Since part of AhR is activated into DNA binding species in the absence of exogenous ligand and competitive AhR antagonist α-naphthoflavone inhibits this activation process with reducing CYP1A1 mRNA expression levels, the presence of endogenous ligand is indicated.
KW - AhR
KW - Endogenous ligand
KW - Human monocytes and macrophages
KW - Physiological function
KW - Transcriptional regulation
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U2 - 10.1023/A:1012762519424
DO - 10.1023/A:1012762519424
M3 - Article
C2 - 11768231
AN - SCOPUS:0035200211
VL - 226
SP - 107
EP - 117
JO - Enzymologia
JF - Enzymologia
SN - 0300-8177
IS - 1-2
ER -