Aryl hydrocarbon receptor/dioxin receptor in human monocytes and macrophages

K. Komura, S. I. Hayashi, I. Makino, L. Poellinger, H. Tanaka

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Aryl hydrocarbon receptor (AhR) belongs to the bHLH/PAS transcription factor family and is activated by various polycyclic or halogenated aromatic hydrocarbons, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3MC). In the present study, we showed that in U937 cells and human macrophages AhR, with its partner cofactor Arnt, is expressed and CYP1A1 mRNA expression is induced in the presence of AhR ligand 3MC. Moreover, we showed that AhR, associating with Arnt, binds to target DNA sequences and activates transcription. Since part of AhR is activated into DNA binding species in the absence of exogenous ligand and competitive AhR antagonist α-naphthoflavone inhibits this activation process with reducing CYP1A1 mRNA expression levels, the presence of endogenous ligand is indicated.

Original languageEnglish
Pages (from-to)107-117
Number of pages11
JournalMolecular and Cellular Biochemistry
Volume226
Issue number1-2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • AhR
  • Endogenous ligand
  • Human monocytes and macrophages
  • Physiological function
  • Transcriptional regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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