Arrest in primitive erythroid cell development caused by promoter- specific disruption of the GATA-1 gene

Satoru Takahashi, Kou Onodera, Hozumi Motohashi, Naruyoshi Suwabe, Norio Hayashi, Nobuaki Yanai, Yoichi Nabesima, Masayuki Yamamoto

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

To elucidate the in vivo function of GATA-1 during hematopoiesis, we specifically disrupted the erythroid promoter of the GATA-1 gene in embryonic stem cells and generated germ line chimeras. Male offspring of chimeras hearing the targeted mutation were found to die by 12.5 days post coitus due to severe anemia while heterozygous females displayed characteristics ranging from severe anemia to normal erythropoiesis. When female heterozygotes were crossed with transgenic males carrying a reporter gene, which specifically marks primitive erythroid progenitors, massive accumulation of undifferentiated erythroid cells were observed in the yolk sacs of the GATA- 1-mutant embryos, demonstrating that GATA-1 is required for the terminal differentiation of primitive erythroid cells in vivo.

Original languageEnglish
Pages (from-to)12611-12615
Number of pages5
JournalJournal of Biological Chemistry
Volume272
Issue number19
DOIs
Publication statusPublished - 1997 May 9

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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