Arrangement of cells within semi-closed space with microelectrode-based technique

Masahiko Hashimoto, Soichiro Sekine, Takeaki Kawashima, Hirokazu Kaji, Matsuhiko Nishizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports strategies for the site-specific immobilization of cells within three-dimensional structures, which are based on a newly developed surface patterning technique, electrochemical bio-lithography. The technique uses a microelectrode to electrochemically generate oxidizing agent that causes local detachment of the anti-biofouling molecules from a substrate surface. Subsequent adsorption of cell-adhesive protein on the newly exposed surface induces local attachment. We combine this technique with dielectrophoresis (DEP) to rapidly collect cells on the cell-adhesive region created in a microfluidic chip. Also, the combination of the electrochemical bio-lithography with layer-by-layer deposition enabled the formation of patterned co-culthres inside elastic tubing structures.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages1318-1320
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: 2007 Oct 72007 Oct 11

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period07/10/707/10/11

Keywords

  • Cell patterning
  • Co-cultivation
  • Dielectrophoresis
  • Electrochemical method

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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