Arf6, Rab11 and transferrin receptor define distinct populations of recycling endosomes

Hotaka Kobayashi, Mitsunori Fukuda

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Recycling endosomes are key platforms for endocytic recycling that return internalized molecules back to the plasma membrane. To determine how recycling endosomes perform their functions, searching for proteins and lipids that specifically localized at recycling endosomes has often been performed by colocalization analyses between candidate molecules and conventional recycling endosome markers. However, it remains unclear whether all the conventional markers have identical localizations. Here we report finding that three well-known recycling endosome markers, i.e., Arf6, Rab11 and transferrin receptor (TfR), have different intracellular localizations in PC12 cells. The results of immunofluorescence analyses showed that the signals of endogenous Arf6, Rab11 and TfR in nerve growth factorstimulated PC12 cells generally differed, although there was some overlapping. Our findings provide new information about recycling endosome markers, and they highlight the heterogeneity of recycling endosomes.

    Original languageEnglish
    Article numbere25036
    JournalCommunicative and Integrative Biology
    Volume6
    Issue number5
    DOIs
    Publication statusPublished - 2013 Oct 2

    Keywords

    • Arf6
    • Rab11
    • Rab35
    • Recycling endosome
    • Transferrin receptor

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)

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