Arachidonic acid-induced channel- and carrier-type ion transport across planar bilayer lipid membranes

Ayumi Hirano, Yuko Namatame, Eiichi Wakaizumi, Yuka Matsuno, Masao Sugawara

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Transmembrane ion transport by arachidonic acid (AA) through bilayer lipid membranes (BLMs) was investigated by means of electrochemical measurements to provide a basis for designing a sensor membrane. We found that AA induces a channel-type current, in addition to a carrier-type current, across planar BLMs. A linear relation between the logarithmic value of the AA concentration and the current responses (given as integrated currents) was observed for a carrier-type current, while a sigmoid relation was found for a channel-type current. Although AA transports Na + , Ca 2+ and Mg 2+ and exhibits ion selectivity between Na + and Mg 2+ for the carrier-type current, ion transport for the channel-type current was non-selective. It was found that ion transport via the channel mechanism occurs frequently for AA, while channel-type currents were only occasionally observed for γ-linolenic acid and prostaglandin D 2 . No channel-type currents were induced by other fatty acids (oleic, linoleic, stearic, myristic, eicosapentanoic and docosahexanoic acids) and metabolites of AA (12-HETE and 5-HETE). The carrier-type ion transport occurs selectively to these compounds if the concentration is below 1.0 μM. These results suggest that AA selectively facilitates an ion flux through the BLMs, generating channel-type and/or carrier-type currents, which can be used as a measure of the AA concentration.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
Journalanalytical sciences
Volume19
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1

ASJC Scopus subject areas

  • Analytical Chemistry

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