Arabidopsis thaliana has a set of J proteins in the endoplasmic reticulum that are conserved from yeast to animals and plants

Masaya Yamamoto, Daisuke Maruyama, Toshiya Endo, Shuh Ichi Nishikawa

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

J domain-containing proteins (J proteins) are functional partners for heat shock protein 70 (Hsp70) molecular chaperones and mediate various cellular processes by regulating activities of Hsp70. Budding yeast has three J proteins in the endoplasmic reticulum (ER): Scj1p and Jem1p functioning in protein folding and quality control in the ER, and Sec63p functioning in protein translocation across the ER membrane as partners for BiP, an Hsp70 in the ER. Here we report that Arabidopsis thaliana has orthologs of these yeast ER J proteins, which we designated as AtERdj3A, AtERdj3B, AtP58IPK, AtERdj2A and AtERdj2B. Tunicamycin treatment of Arabidopsis cells, which causes ER stress, led to up-regulation of AtERdj3A, AtERdj3B, AtP58IPK and AtERdj2B. Subcellular fractionation analyses showed their ER localization, indicating that the identified J proteins indeed function as partners for BiP in Arabidopsis cells. Since expression of AtERdj3A, AtERdj3B and AtP58 IPK partially suppressed the growth defects of the yeast jem1Δscj1Δ mutant, they have functions similar to those of Scj1p and Jem1p. T-DNA insertions of the AtERDJ2A gene resulted in pollen germination defects, probably reflecting its essential function in protein translocation. These results suggest that A. thaliana has a set of ER J proteins structurally and functionally conserved from yeast to plants.

Original languageEnglish
Pages (from-to)1547-1562
Number of pages16
JournalPlant and Cell Physiology
Volume49
Issue number10
DOIs
Publication statusPublished - 2008 Oct 1
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • Endoplasmic reticulum
  • J proteins
  • Molecular chaperone
  • Quality control

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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