Arabidopsis regenerating protoplast: A powerful model system for combining the proteomics of cellwall proteins and the visualization of cell wall dynamics

Ryusuke Yokoyama, Hiroaki Kuki, Takeshi Kuroha, Kazuhiko Nishitani

    Research output: Contribution to journalReview articlepeer-review

    7 Citations (Scopus)

    Abstract

    The development of a range of sub-proteomic approaches to the plant cell wall has identified many of the cell wall proteins. However, it remains difficult to elucidate the precise biological role of each protein and the cell wall dynamics driven by their actions. The plant protoplast provides an excellent means not only for characterizing cell wall proteins, but also for visualizing the dynamics of cell wall regeneration, during which cell wall proteins are secreted. It therefore offers a unique opportunity to investigate the de novo construction process of the cell wall. This review deals with sub-proteomic approaches to the plant cell wall through the use of protoplasts, a methodology that will provide the basis for further exploration of cell wall proteins and cell wall dynamics.

    Original languageEnglish
    Article number34
    JournalProteomes
    Volume4
    Issue number4
    DOIs
    Publication statusPublished - 2016 Dec 1

    Keywords

    • Cell wall
    • Imaging technique
    • Protoplast
    • Sub-proteomics

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Clinical Biochemistry

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