Arabidopsis mutant plants with diverse defects in polyamine metabolism show unequal sensitivity to exogenous cadaverine probably based on their spermine content

Taibo Liu, Hayato Dobashi, Dong Wook Kim, G. H.M. Sagor, Masaru Niitsu, Thomas Berberich, Tomonobu Kusano

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Arabidopsis plants do not synthesize the polyamine cadaverine, a five carbon-chain diamine and structural analog of putrescine. Mutants defective in polyamine metabolic genes were exposed to exogenous cadaverine. Spermine-deficient spms mutant grew well while a T-DNA insertion mutant (pao4-1) of polyamine oxidase (PAO) 4 was severely inhibited in root growth compared to wild type (WT) or other pao loss-of-function mutants. To understand the molecular basis of this phenomenon, polyamine contents of WT, spms and pao4-1 plants treated with cadaverine were analyzed. Putrescine contents increased in all the three plants, and spermidine contents decreased in WT and pao4-1 but not in spms. Spermine contents increased in WT and pao4-1. As there were good correlations between putrescine (or spermine) contents and the degree of root growth inhibition, effects of exogenously added putrescine and spermine were examined. Spermine mimicked the original phenomenon, whereas high levels of putrescine evenly inhibited root growth, suggesting that cadaverine-induced spermine accumulation may explain the phenomenon. We also tested growth response of cadaverine-treated WT and pao4-1 plants to NaCl and found that spermine-accumulated pao4-1 plant was not NaCl tolerant. Based on the results, the effect of cadaverine on Arabidopsis growth and the role of PAO during NaCl stress are discussed.

    Original languageEnglish
    Pages (from-to)151-159
    Number of pages9
    JournalPhysiology and Molecular Biology of Plants
    Volume20
    Issue number2
    DOIs
    Publication statusPublished - 2014 Apr 1

    Keywords

    • Arabidopsis
    • Cadaverine
    • NaCl response
    • Polyamine oxidase
    • Putrescine
    • Spermidine
    • Spermine

    ASJC Scopus subject areas

    • Physiology
    • Molecular Biology
    • Plant Science

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