APPLICATION OF THE MICROPIPETTE TECHNIQUE TO THE MEASUREMENT OF ENDOTHELIAL CELL MECHANICAL PROPERTIES.

M. Sato, M. J. Levesque, R. M. Nerem

Research output: Contribution to journalConference articlepeer-review

3 Citations (Scopus)

Abstract

The mechanical properties of the endothelial cell were studied using the micropipette technique. Bovine aortic endothelial cells cultured in the laboratory on Thermanox plastic cover slips were used. Fully confluent cultured endothelial cell populations from 7th to 9th generation were studied both for static, no flow conditions and after exposure to a steady shear stress using a parallel plate, channel flow device. Endothelial cells were exposed to static, no flow conditions or a specific shear stress (10, 30, or 85 dynes/cm**2) for a duration of 0. 5 to 24 hours. After exposure, endothelial cells were detached from their substrate either by trypsin or a mechanical method and then suspended in MDM. Such an isolated, suspended cell is then approached by a small micropipette (inside radius, R congruent 1. 5 mu m), which is attached to a pressure reservoir. The elongation of the cell membrane inside the micropipette, L, is recorded as a function of pressure, DELTA p. This in effect constitutes a stress-strain measurement.

Original languageEnglish
Pages (from-to)167-169
Number of pages3
JournalAmerican Society of Mechanical Engineers, Applied Mechanics Division, AMD
Volume68
Publication statusPublished - 1985 Jan 1

ASJC Scopus subject areas

  • Mechanical Engineering

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