The mechanical properties of the endothelial cell were studied using the micropipette technique. Bovine aortic endothelial cells cultured in the laboratory on Thermanox plastic cover slips were used. Fully confluent cultured endothelial cell populations from 7th to 9th generation were studied both for static, no flow conditions and after exposure to a steady shear stress using a parallel plate, channel flow device. Endothelial cells were exposed to static, no flow conditions or a specific shear stress (10, 30, or 85 dynes/cm**2) for a duration of 0. 5 to 24 hours. After exposure, endothelial cells were detached from their substrate either by trypsin or a mechanical method and then suspended in MDM. Such an isolated, suspended cell is then approached by a small micropipette (inside radius, R congruent 1. 5 mu m), which is attached to a pressure reservoir. The elongation of the cell membrane inside the micropipette, L, is recorded as a function of pressure, DELTA p. This in effect constitutes a stress-strain measurement.
|Number of pages||3|
|Journal||American Society of Mechanical Engineers, Applied Mechanics Division, AMD|
|Publication status||Published - 1985 Jan 1|
ASJC Scopus subject areas
- Mechanical Engineering