Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties

M. Sato, D. P. Theret, L. T. Wheeler, N. Ohshima, R. M. Nerem

Research output: Contribution to journalArticlepeer-review

315 Citations (Scopus)

Abstract

The viscoelastic deformation of porcine aortic endothelial cells grown under static culture conditions was measured using the micropipette technique. Experiments were conducted both for control cells (mechanically or trypsin detached from the substrate) and for cells in which cytoskeletal elements were disrupted by cytochalasin B or colchicine. The time course of the aspirated length into the pipette was measured after applying a stepwise increase in aspiration pressure. To analyze the data, a standard linear viscoelastic half-space model of the endothelial cell was used. The aspirated length was expressed as an exponential function of time. The actin microfilaments were found to be the major cytoskeletal component determining the viscoelastic response of endothelial cells grown in static culture.

Original languageEnglish
Pages (from-to)263-268
Number of pages6
JournalJournal of Biomechanical Engineering
Volume112
Issue number3
DOIs
Publication statusPublished - 1990 Aug

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

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