TY - JOUR
T1 - Application of the fluctuation theorem to motor proteins
T2 - from F1-ATPase to axonal cargo transport by kinesin and dynein
AU - Hayashi, Kumiko
N1 - Funding Information:
Funding information This work was supported by grants (grant numbers JP17gm5810009 and JP17ja0110011) from the Japan Agency for Medical Research and Development (AMED) and a Grants-in-Aid for Scientific Research (KAKENHI) (grant numbers 26104501, 26115702, 26310204, and 16H00819) from the Ministry of Education, Culture, Sports, Science, and Technology.
Funding Information:
We thank the participants of the 2017 annual meeting of the Australian Society of Biophysics for comments on the study.
Publisher Copyright:
© 2018, International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - The fluctuation theorem is a representative theorem in non-equilibrium statistical physics actively studied in the 1990s. Relating to entropy production in non-equilibrium states, the theorem has been used to estimate the driving power of motor proteins from fluctuation in their motion. In this review, usage of the fluctuation theorem in experiments on motor proteins is illustrated for biologists, especially those who study mechanobiology, in which force measurement is a central issue. We first introduce the application of the fluctuation theorem in measuring the rotary torque of the rotary motor protein F1-ATPase. Next, as an extension of this application, a recent trial estimating the force generated during cargo transport in vivo by the microtubule motors kinesin and dynein is introduced. Elucidation of the physical mechanism of such transport is important, especially for neurons, in which deficits in cargo transport are deeply related to neuronal diseases. Finally, perspectives on the fluctuation theorem as a new technique in the field of neuroscience are discussed.
AB - The fluctuation theorem is a representative theorem in non-equilibrium statistical physics actively studied in the 1990s. Relating to entropy production in non-equilibrium states, the theorem has been used to estimate the driving power of motor proteins from fluctuation in their motion. In this review, usage of the fluctuation theorem in experiments on motor proteins is illustrated for biologists, especially those who study mechanobiology, in which force measurement is a central issue. We first introduce the application of the fluctuation theorem in measuring the rotary torque of the rotary motor protein F1-ATPase. Next, as an extension of this application, a recent trial estimating the force generated during cargo transport in vivo by the microtubule motors kinesin and dynein is introduced. Elucidation of the physical mechanism of such transport is important, especially for neurons, in which deficits in cargo transport are deeply related to neuronal diseases. Finally, perspectives on the fluctuation theorem as a new technique in the field of neuroscience are discussed.
KW - Cellular cargo transport
KW - Fluctuation theorem
KW - Mechanobiology
KW - Motor proteins
KW - Neuronal disease
KW - Non-invasive force measurement
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U2 - 10.1007/s12551-018-0440-5
DO - 10.1007/s12551-018-0440-5
M3 - Review article
AN - SCOPUS:85056611969
VL - 10
SP - 1311
EP - 1321
JO - Biophysical Reviews
JF - Biophysical Reviews
SN - 1867-2450
IS - 5
ER -