TY - JOUR
T1 - Application of the chemical imaging sensor to electrophysiological measurement of a neural cell
AU - Tanaka, Hiroshi
AU - Yoshinobu, Tatsuo
AU - Iwasaki, Hiroshi
N1 - Funding Information:
The authors are grateful to Prof. C. Bessho at Kyoto Sangyo University and Prof. M. Sakakibara at Tokai University for their advice and offer of facilities and specimens. Animals were supplied also from the Aplysia Resource Facility at the University of Miami's Rosenstiel School of Marine and Atmospheric Sciences (RMAS). The authors are also grateful to Mr. H. Sugihara at Matsushita Electric Industrial for his advice. Si wafers used in this study were by courtesy of Horiba and Matsushita Electric Industrial. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture (contract nos. 09359005 and 09650890).
PY - 1999/10/5
Y1 - 1999/10/5
N2 - The chemical imaging sensor is applied to electrophysiological measurement of a neural cell. In this method, neural cells are cultured directly on the sensing surface, and a laser beam is used to specify a cell to be measured. The amplitude of the ac photocurrent induced by the laser beam is measured to detect the change of the membrane potential of the cell. In this study, the membrane potential of the cell is varied by injection of a dc current, and it is demonstrated that the ac photocurrent signal responds to the membrane potential. This method would enable noninvasive electric measurement of neural cells, networks or tissues under long-term culture.
AB - The chemical imaging sensor is applied to electrophysiological measurement of a neural cell. In this method, neural cells are cultured directly on the sensing surface, and a laser beam is used to specify a cell to be measured. The amplitude of the ac photocurrent induced by the laser beam is measured to detect the change of the membrane potential of the cell. In this study, the membrane potential of the cell is varied by injection of a dc current, and it is demonstrated that the ac photocurrent signal responds to the membrane potential. This method would enable noninvasive electric measurement of neural cells, networks or tissues under long-term culture.
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U2 - 10.1016/S0925-4005(99)00012-X
DO - 10.1016/S0925-4005(99)00012-X
M3 - Article
AN - SCOPUS:0033318102
VL - 59
SP - 21
EP - 25
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 1
ER -