Application of spin-trapping study to rat ischemic brain homogenate incubated with NADPH and Fe-EDTA

Teiji Tominaga, Shigeki Imaizumi, Takahashi Yoshimoto, Jiro Suzuki, Yuzo Fujita

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

A spin-trapping technique was applied to the detection of free radicals generated in nicotinamide-adenine denucleotide phosphate (NADPH)-dependent lipid peroxidation of rat ischemic brain homogenate. The spin adduct of phenyl-t-butylnitrone if(AN = 16.2-16.5 g, Aβ H = 3.6-3.8 g) was observed, and it was thought to be derived from NADPH-dependent lipid peroxidation from its oxygen and NADPH dependency. Its intensity was increased in the recirculated condition following ischemic insult, indicating the susceptibility of brain tissue to lipid peroxidation in such a condition.

Original languageEnglish
Pages (from-to)370-372
Number of pages3
JournalBrain research
Volume402
Issue number2
DOIs
Publication statusPublished - 1987 Feb 3

Keywords

  • Brain ischemia
  • Electron spin resonance
  • Free radical
  • Lipid peroxidation
  • Spin trapping

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Application of spin-trapping study to rat ischemic brain homogenate incubated with NADPH and Fe-EDTA'. Together they form a unique fingerprint.

Cite this