Application of spin-trapping study to rat ischemic brain homogenate incubated with NADPH and Fe-EDTA

Teiji Tominaga; Shigeki Imaizumi; Takahashi Yoshimoto; Jiro Suzuki; Yuzo Fujita

doi:10.1016/0006-8993(87)90048-5

Application of spin-trapping study to rat ischemic brain homogenate incubated with NADPH and Fe-EDTA

Teiji Tominaga, Shigeki Imaizumi, Takahashi Yoshimoto, Jiro Suzuki, Yuzo Fujita

University Hospital (not affiliated with any section)

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A spin-trapping technique was applied to the detection of free radicals generated in nicotinamide-adenine denucleotide phosphate (NADPH)-dependent lipid peroxidation of rat ischemic brain homogenate. The spin adduct of phenyl-t-butylnitrone if(A_N = 16.2-16.5 g, A_β ^{H = 3.6-3.8 g}) was observed, and it was thought to be derived from NADPH-dependent lipid peroxidation from its oxygen and NADPH dependency. Its intensity was increased in the recirculated condition following ischemic insult, indicating the susceptibility of brain tissue to lipid peroxidation in such a condition.

Original language	English
Pages (from-to)	370-372
Number of pages	3
Journal	Brain research
Volume	402
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(87)90048-5
Publication status	Published - 1987 Feb 3

Keywords

Brain ischemia
Electron spin resonance
Free radical
Lipid peroxidation
Spin trapping

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(87)90048-5

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title = "Application of spin-trapping study to rat ischemic brain homogenate incubated with NADPH and Fe-EDTA",

abstract = "A spin-trapping technique was applied to the detection of free radicals generated in nicotinamide-adenine denucleotide phosphate (NADPH)-dependent lipid peroxidation of rat ischemic brain homogenate. The spin adduct of phenyl-t-butylnitrone if(AN = 16.2-16.5 g, Aβ H = 3.6-3.8 g) was observed, and it was thought to be derived from NADPH-dependent lipid peroxidation from its oxygen and NADPH dependency. Its intensity was increased in the recirculated condition following ischemic insult, indicating the susceptibility of brain tissue to lipid peroxidation in such a condition.",

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author = "Teiji Tominaga and Shigeki Imaizumi and Takahashi Yoshimoto and Jiro Suzuki and Yuzo Fujita",

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AU - Tominaga, Teiji

AU - Imaizumi, Shigeki

AU - Yoshimoto, Takahashi

AU - Suzuki, Jiro

AU - Fujita, Yuzo

PY - 1987/2/3

Y1 - 1987/2/3

N2 - A spin-trapping technique was applied to the detection of free radicals generated in nicotinamide-adenine denucleotide phosphate (NADPH)-dependent lipid peroxidation of rat ischemic brain homogenate. The spin adduct of phenyl-t-butylnitrone if(AN = 16.2-16.5 g, Aβ H = 3.6-3.8 g) was observed, and it was thought to be derived from NADPH-dependent lipid peroxidation from its oxygen and NADPH dependency. Its intensity was increased in the recirculated condition following ischemic insult, indicating the susceptibility of brain tissue to lipid peroxidation in such a condition.

AB - A spin-trapping technique was applied to the detection of free radicals generated in nicotinamide-adenine denucleotide phosphate (NADPH)-dependent lipid peroxidation of rat ischemic brain homogenate. The spin adduct of phenyl-t-butylnitrone if(AN = 16.2-16.5 g, Aβ H = 3.6-3.8 g) was observed, and it was thought to be derived from NADPH-dependent lipid peroxidation from its oxygen and NADPH dependency. Its intensity was increased in the recirculated condition following ischemic insult, indicating the susceptibility of brain tissue to lipid peroxidation in such a condition.

KW - Brain ischemia

KW - Electron spin resonance

KW - Free radical

KW - Lipid peroxidation

KW - Spin trapping

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Application of spin-trapping study to rat ischemic brain homogenate incubated with NADPH and Fe-EDTA

Abstract

Keywords

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