Application of response surface methodology in the optimisation of a growth medium for enhanced natural preservative bacteriocin production by a probiotic bacterium

Paulraj Kanmani, R. Satish Kumar, N. Yuvaraj, K. A. Paari, V. Pattukumar, Venkatesan Arul

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    In this study, a statistics-based experimental design was utilised for the optimisation of a growth medium which possibly enhanced bacteriocin production by Streptococcus phocae PI80. Carbon, nitrogen sources and a bio-surfactant were first screened using a one variable at a time technique and scored for increasing yield production. The selected variables were further statistically optimised using response surface methodology with a central composite design. The high-and low-level limits of the selected variables were determined, and a set of 34 experimental runs were performed. The concentration of each medium ingredient influenced the bacteriocin activity to about 22,500AUmL-1. The carbon and nitrogen sources were identified as significant factors in restraining the bacteriocin activity produced by S. phocae PI80. The statistics-based experimental design was found to be very efficient in optimising the media components in a number of experimental runs, with a three-fold increase in bacteriocin activity compared to the un-optimised medium. The optimum medium composition was found to be sodium succinate (10.0gL -1), yeast extract (4.0gL-1), glucose (9.0gL -1), NaCl (10.0gL-1), Tween 80 (6.0gL-1) and K2HPO4(1.0gL1). This optimised medium is two-fold more cost effective than the commercial Lactobacillus MRS medium.

    Original languageEnglish
    Pages (from-to)1539-1543
    Number of pages5
    JournalNatural Product Research
    Volume26
    Issue number16
    DOIs
    Publication statusPublished - 2012 Aug 1

    Keywords

    • Streptococcus phocae
    • bacteriocin
    • natural biopreservative
    • optimisation
    • response surface methodology

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Plant Science
    • Organic Chemistry

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