TY - GEN
T1 - Application of polyvinyl alcohol (PVA) macro-encapsulated islets in islet transplantation
AU - Qi, Zhi
AU - Qi, Meirigeng
AU - Sakata, Naoaki
AU - Yamamoto, Chizuru
AU - Yanai, Goichi
AU - Ikenoue, Etsuko
AU - Wu, Qian
AU - Hiura, Akihito
AU - Sumi, Shoichiro
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2009
Y1 - 2009
N2 - Background: Islet transplantation has shown great success in the treatment of type 1 diabetes. However, it still has two major problems to overcome, namely donor shortage and the side effects of immunosuppression. Encapsulated islets have emerged as a potential option on islet transplantation because it can, at least partly, overcome these two problems. Methods: After making PVA macro-encapsulated islets, recovery rate, insulin content, insulin secretion test, and morphological changes in culture with/without fresh human plasma were evaluated in vitro. PVA macro-encapsulated rat islets were transplanted into the peritoneal cavity of STZ-diabetic mice and rats. We observed blood glucose, body weight, survival rate, blood urea nitrogen (BUN), creatinine (Cr), and performed histological assessment in vivo. Results: After 14-day culture, the encapsulated islets, maintained a normal morphology and better insulin secretion in response to high glucose. Addition of fresh human plasma into the culture medium destroyed free islets in 48 hours but did not affect the morphology and insulin content of PVA macro-encapsulated islets. After transplantation to diabetic mice and rats, encapsulated islets decreased blood glucose levels, prevented weight loss and improved the survival rate in comparison to the negative control groups. The BUN and Cr levels were also decreased significantly after 1 week in the PVA group with amelioration of ketosis. Conclusions: PVA macro-encapsulated islets can protect islets against discordant immunorejection, and ameliorate hyperglycemia, ketosis and diabetic renal dysfunction in STZ induced diabetic mice and rats.
AB - Background: Islet transplantation has shown great success in the treatment of type 1 diabetes. However, it still has two major problems to overcome, namely donor shortage and the side effects of immunosuppression. Encapsulated islets have emerged as a potential option on islet transplantation because it can, at least partly, overcome these two problems. Methods: After making PVA macro-encapsulated islets, recovery rate, insulin content, insulin secretion test, and morphological changes in culture with/without fresh human plasma were evaluated in vitro. PVA macro-encapsulated rat islets were transplanted into the peritoneal cavity of STZ-diabetic mice and rats. We observed blood glucose, body weight, survival rate, blood urea nitrogen (BUN), creatinine (Cr), and performed histological assessment in vivo. Results: After 14-day culture, the encapsulated islets, maintained a normal morphology and better insulin secretion in response to high glucose. Addition of fresh human plasma into the culture medium destroyed free islets in 48 hours but did not affect the morphology and insulin content of PVA macro-encapsulated islets. After transplantation to diabetic mice and rats, encapsulated islets decreased blood glucose levels, prevented weight loss and improved the survival rate in comparison to the negative control groups. The BUN and Cr levels were also decreased significantly after 1 week in the PVA group with amelioration of ketosis. Conclusions: PVA macro-encapsulated islets can protect islets against discordant immunorejection, and ameliorate hyperglycemia, ketosis and diabetic renal dysfunction in STZ induced diabetic mice and rats.
KW - Islet transplantation
KW - Macro-encapsulated islets, type 1 diabetes
KW - Polyvinyl alcohol (PVA)
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U2 - 10.1109/ICBBE.2009.5162392
DO - 10.1109/ICBBE.2009.5162392
M3 - Conference contribution
AN - SCOPUS:72749090384
SN - 9781424429028
T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
T2 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Y2 - 11 June 2009 through 13 June 2009
ER -