Application of planar chromatography to the determination of continine in urine of active and passive smoking pregnant women

Toshimasa Toyo'oka, Dongri Jin, Nobue Tomoi, Tomoyuki Oe, Hidenori Hiranuma

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A quick and economical method for the determination of nicotine and its main metabolite-cotinine in urine from smoking and passively smoking pregnant women is proposed using TLC-densitometry. Chromatographic separations were performed on the plates coated with silica gel bound with C-18 alkyl chains. Chromatograms were developed using a mixture of acetonitrile and water (88 + 12, v/v) in a horizontal chamber. Visualization was carried out under UV illumination at λ = 254 nm and then cotinine was quantified by the use of densitometer in reflectance mode at λ = 260 nm. Nicotine was identified after derivatization with Dragendorff reagent under visible light. The results of nicotine and cotinine analysis were verified by questionnaires obtained from pregnant women according to their age and smoking status correlated with spontaneous abortion frequency and child death rate.

Original languageEnglish
Pages (from-to)50-55
Number of pages6
JournalBiomedical Chromatography
Volume15
Issue number1
DOIs
Publication statusPublished - 2001 Mar 3
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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