Application of nanosecond pulsed electric fields into HeLa cells expressing enhanced green fluorescent protein and fluorescence lifetime microscopy

Kamlesh Awasthi, Takakazu Nakabayashi, Nobuhiro Ohta

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An electrode microchamber has been constructed for applying nanosecond pulsed strong electric fields to living cells, and fluorescence lifetime microscopy (FLIM) has been used to investigate the effects of external electric fields on dynamics and function of HeLa cells expressing enhanced green fluorescent protein (EGFP). Both morphological change in cells and reduction of the fluorescence lifetime of EGFP have been observed after application of electric fields having a pulsed width of 50 ns and a strength of 4 MV m -1, indicating that apoptosis, which is a programmed cell death, was induced by nanosecond pulsed electric fields and that fluorescence lifetime of EGFP decreased along with the induction of apoptosis. The reduction of the fluorescence lifetime occurred before the morphological change, indicating that FLIM provides a sensitive and noninvasive detection of the progress of apoptosis induced by application of nanosecond pulsed electric fields.

Original languageEnglish
Pages (from-to)11159-11165
Number of pages7
JournalJournal of Physical Chemistry B
Volume116
Issue number36
DOIs
Publication statusPublished - 2012 Sep 13
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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