Application of modular kinetic analysis to mitochondrial oxidative phosphorylation in skeletal muscle of birds exposed to acute heat stress

Motoi Kikusato, Jon J. Ramsey, Taku Amo, Masaaki Toyomizu

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (ΔΨ). To clarify the mechanism underlying the elevation of ΔΨ, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of heat-stressed birds (34°C for 12. h). In the birds exposed to heat stress, 'substrate oxidation' (a ΔΨ-producer) was increased compared to control (24°C) birds, although there was little difference in 'proton leak' (a ΔΨ-consumer), suggesting that an elevation in the ΔΨ at state 4 may be due to enhanced substrate oxidation. It thus appears that enhanced substrate oxidation plays a crucial role in the overproduction of ROS for heat-stressed birds, probably via elevated ΔΨ.

    Original languageEnglish
    Pages (from-to)3143-3148
    Number of pages6
    JournalFEBS Letters
    Volume584
    Issue number14
    DOIs
    Publication statusPublished - 2010 Jul

    Keywords

    • Coupling efficiency
    • Heat stress
    • Membrane potential (ΔΨ)
    • Modular kinetic analysis
    • Reactive oxygen species
    • Substrate oxidation

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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