Application of microbial chip for amperometric detection of metabolic alteration in bacteria

Kuniaki Nagamine, Takatoshi Kaya, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The microbial chip of 3.5 nL-volume of collagen gel matrixes entrapping the bacterial cells in living state has been applied to investigate the bacterial response to high osmotic stress. Scanning electrochemical microscopy (SECM) was used to monitor the Fe(CN)64- production that reflects the electron flow at respiratory chain located within the cytoplasmic membrane of bacteria. In the present study, we have found that the ferricyanide reduction activity in the Staphylococcus aureus, osmotolerant bacteria, is enhanced in the presence of highly concentrated salts. This result suggests that the osmotic stress causes the change in membrane structure or membrane-associated protein profile so as to enhance the cell membrane permeability to hydrophilic mediator. The low current responsibility of S. aureus can be improved not only by (1) using hydrophobic mediator, such as quinone derivatives instead of Fe(CN) 63- but also (2) exposing S. aureus cells to high osmotic stress in the presence of hydrophilic mediator.

Original languageEnglish
Pages (from-to)676-682
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume108
Issue number1-2 SPEC. ISS.
DOIs
Publication statusPublished - 2005 Jul 22

Keywords

  • High osmotic stress
  • Microbial chip
  • Scanning electrochemical microscopy
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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