Application of massively parallel pyrosequencing to the discovery of non-dinucleotide microsatellites: Tetra- and pentanucleotide repeat markers for the Pacific abalone (Haliotis discus hannai)

Masashi Sekino; Yuya Shigenobu; Yoji Nakamura; Takuma Sugaya; Motoyuki Hara

doi:10.1007/s12686-012-9678-6

Application of massively parallel pyrosequencing to the discovery of non-dinucleotide microsatellites: Tetra- and pentanucleotide repeat markers for the Pacific abalone (Haliotis discus hannai)

Masashi Sekino, Yuya Shigenobu, Yoji Nakamura, Takuma Sugaya, Motoyuki Hara

Agricultural Science

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The accelerated progress of ultra high-throughput DNA sequencing technologies has facilitated the process of identifying microsatellites in the genomes, attenuating laborious experimental procedures involved in traditional approaches. We applied a massively parallel pyrosequencing technique to the development of tetra- and pentanucleotide microsatellite markers for the Pacific abalone Haliotis discus hannai.

Original language	English
Pages (from-to)	939-941
Number of pages	3
Journal	Conservation Genetics Resources
Volume	4
Issue number	4
DOIs	https://doi.org/10.1007/s12686-012-9678-6
Publication status	Published - 2012 Dec 1

Keywords

Next-generation sequencing
Short tandem repeat
Simple sequence repeat

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-012-9678-6

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Application of massively parallel pyrosequencing to the discovery of non-dinucleotide microsatellites : Tetra- and pentanucleotide repeat markers for the Pacific abalone (Haliotis discus hannai). / Sekino, Masashi; Shigenobu, Yuya; Nakamura, Yoji; Sugaya, Takuma; Hara, Motoyuki.

In: Conservation Genetics Resources, Vol. 4, No. 4, 01.12.2012, p. 939-941.

Research output: Contribution to journal › Article › peer-review

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