TY - JOUR
T1 - Application of massively parallel pyrosequencing to the discovery of non-dinucleotide microsatellites
T2 - Tetra- and pentanucleotide repeat markers for the Pacific abalone (Haliotis discus hannai)
AU - Sekino, Masashi
AU - Shigenobu, Yuya
AU - Nakamura, Yoji
AU - Sugaya, Takuma
AU - Hara, Motoyuki
PY - 2012/12
Y1 - 2012/12
N2 - The accelerated progress of ultra high-throughput DNA sequencing technologies has facilitated the process of identifying microsatellites in the genomes, attenuating laborious experimental procedures involved in traditional approaches. We applied a massively parallel pyrosequencing technique to the development of tetra- and pentanucleotide microsatellite markers for the Pacific abalone Haliotis discus hannai.
AB - The accelerated progress of ultra high-throughput DNA sequencing technologies has facilitated the process of identifying microsatellites in the genomes, attenuating laborious experimental procedures involved in traditional approaches. We applied a massively parallel pyrosequencing technique to the development of tetra- and pentanucleotide microsatellite markers for the Pacific abalone Haliotis discus hannai.
KW - Next-generation sequencing
KW - Short tandem repeat
KW - Simple sequence repeat
UR - http://www.scopus.com/inward/record.url?scp=84867583579&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867583579&partnerID=8YFLogxK
U2 - 10.1007/s12686-012-9678-6
DO - 10.1007/s12686-012-9678-6
M3 - Article
AN - SCOPUS:84867583579
VL - 4
SP - 939
EP - 941
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
SN - 1877-7252
IS - 4
ER -