Application of massively parallel pyrosequencing to the discovery of non-dinucleotide microsatellites: Tetra- and pentanucleotide repeat markers for the Pacific abalone (Haliotis discus hannai)

Masashi Sekino, Yuya Shigenobu, Yoji Nakamura, Takuma Sugaya, Motoyuki Hara

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The accelerated progress of ultra high-throughput DNA sequencing technologies has facilitated the process of identifying microsatellites in the genomes, attenuating laborious experimental procedures involved in traditional approaches. We applied a massively parallel pyrosequencing technique to the development of tetra- and pentanucleotide microsatellite markers for the Pacific abalone Haliotis discus hannai.

Original languageEnglish
Pages (from-to)939-941
Number of pages3
JournalConservation Genetics Resources
Volume4
Issue number4
DOIs
Publication statusPublished - 2012 Dec 1

Keywords

  • Next-generation sequencing
  • Short tandem repeat
  • Simple sequence repeat

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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