Application of 13C NMR spectroscopy to characterize organic chemical components of decomposing coarse woody debris from different climatic regions

T. Hishinuma, T. Osono, Y. Fukasawa, Jun Ichi Azuma, H. Takeda

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    7 Citations (Scopus)

    Abstract

    Solid-state 13C nuclear magnetic resonance (NMR) spectroscopy was applied to coarse woody debris (CWD) in different stages of decomposition and collected from forest floor of a subtropical, a cool temperate, and a subalpine forest in Japan. The purpose was to test its applicability to characterize organic chemical composition of CWD of broad-leaved and coniferous trees from different climatic conditions. O-alkyl-C, mainly representing carbohydrates, was the predominant component of CWD at the three sites, accounting for 43.5-58.1% of the NMR spectra. Generally, the relative area under the signals for aromatic-C and phenolic-C, mainly representing lignin, increased, whereas the relative area for O-alkyl-C decreased, as the decay class advanced. The relative area under NMR chemical shift regions was significantly correlated with the chemical properties examined with proximate analyses. That is, O-alkyl-C and di-O-alkyl-C NMR signal areas were positively correlated with the volumetric density of CWD and the content of total carbohydrates. Methoxyl-C, aromatic-C, phenolic-C, carboxyl-C, and carbonyl-C were positively correlated with the contents of acid-unhydrolyzable residues (lignin, tannins, and cutin) and nitrogen. Lignin-C calculated from NMR signals increased, and polysaccharide-C decreased, with the decay class of CWD at the three study sites. A review of previous studies on 13C NMR spectroscopy for decomposing CWD suggested further needs of its application to broad-leaved trees from tropical and subtropical regions. Keywords Carbon-13 nuclear magnetic resonance spectroscopy, coarse woody debris, lignin, decomposition, wood.

    Original languageEnglish
    Pages (from-to)3-13
    Number of pages11
    JournalAnnals of Forest Research
    Volume58
    Issue number1
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Carbon-13 nuclear magnetic resonance spectroscopy
    • Coarse woody debris
    • Decomposition
    • Lignin
    • Wood

    ASJC Scopus subject areas

    • Forestry
    • Ecology
    • Plant Science

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