Abstract
Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.
Original language | English |
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Pages (from-to) | 122-125 |
Number of pages | 4 |
Journal | Fisheries Science |
Volume | 62 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1996 Feb |
Keywords
- Distribution
- Isoelectric focusing
- Multiple enzyme forms
- Opine
- Opine dehydrogenase
- Substrate specificity
ASJC Scopus subject areas
- Aquatic Science