Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates

Nobuhiro Kanno, Kunihide Kamimura, Eizou Nagahisa, Minoru Sato, Yoshikazu Sato

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.

Original languageEnglish
Pages (from-to)122-125
Number of pages4
JournalFisheries Science
Volume62
Issue number1
DOIs
Publication statusPublished - 1996 Feb

Keywords

  • Distribution
  • Isoelectric focusing
  • Multiple enzyme forms
  • Opine
  • Opine dehydrogenase
  • Substrate specificity

ASJC Scopus subject areas

  • Aquatic Science

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