Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates

Nobuhiro Kanno; Kunihide Kamimura; Eizou Nagahisa; Minoru Sato; Yoshikazu Sato

doi:10.2331/fishsci.62.122

Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates

Nobuhiro Kanno, Kunihide Kamimura, Eizou Nagahisa, Minoru Sato, Yoshikazu Sato

AGR - Biological Resource Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.

Original language	English
Pages (from-to)	122-125
Number of pages	4
Journal	Fisheries Science
Volume	62
Issue number	1
DOIs	https://doi.org/10.2331/fishsci.62.122
Publication status	Published - 1996 Feb

Keywords

Distribution
Isoelectric focusing
Multiple enzyme forms
Opine
Opine dehydrogenase
Substrate specificity

ASJC Scopus subject areas

Aquatic Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2331/fishsci.62.122

Cite this

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title = "Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates",

abstract = "Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.",

keywords = "Distribution, Isoelectric focusing, Multiple enzyme forms, Opine, Opine dehydrogenase, Substrate specificity",

author = "Nobuhiro Kanno and Kunihide Kamimura and Eizou Nagahisa and Minoru Sato and Yoshikazu Sato",

year = "1996",

month = feb,

doi = "10.2331/fishsci.62.122",

language = "English",

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journal = "Fisheries Science",

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T1 - Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates

AU - Kanno, Nobuhiro

AU - Kamimura, Kunihide

AU - Nagahisa, Eizou

AU - Sato, Minoru

AU - Sato, Yoshikazu

PY - 1996/2

Y1 - 1996/2

N2 - Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.

AB - Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.

KW - Distribution

KW - Isoelectric focusing

KW - Multiple enzyme forms

KW - Opine

KW - Opine dehydrogenase

KW - Substrate specificity

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DO - 10.2331/fishsci.62.122

M3 - Article

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VL - 62

SP - 122

EP - 125

JO - Fisheries Science

JF - Fisheries Science

SN - 0919-9268

IS - 1

ER -

Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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