Abstract
The heat shock promoter is useful for regulating transgene expression in small water-living organisms. In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. One such way for inducing heat shock response in targeted cells is by using a laser microbeam under the microscope. In addition, random mosaic expression by transient gene expression and transplantation of the transgenic embryo into a wild type host can be considered a powerful tool for studying gene functions using this promoter. In this paper, we review the applications of the zebrafish heat shock protein promoter as a gene expression tool and for lineage labeling and transcription enhancer screening.
Original language | English |
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Pages (from-to) | 401-406 |
Number of pages | 6 |
Journal | Development Growth and Differentiation |
Volume | 50 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2008 Aug |
Externally published | Yes |
Keywords
- Transgenic
- Zebrafish
- hsp70
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology