Application of heat shock promoter in transgenic zebrafish

Wataru Shoji, Mika Sato-Maeda

Research output: Contribution to journalReview articlepeer-review

50 Citations (Scopus)

Abstract

The heat shock promoter is useful for regulating transgene expression in small water-living organisms. In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. One such way for inducing heat shock response in targeted cells is by using a laser microbeam under the microscope. In addition, random mosaic expression by transient gene expression and transplantation of the transgenic embryo into a wild type host can be considered a powerful tool for studying gene functions using this promoter. In this paper, we review the applications of the zebrafish heat shock protein promoter as a gene expression tool and for lineage labeling and transcription enhancer screening.

Original languageEnglish
Pages (from-to)401-406
Number of pages6
JournalDevelopment Growth and Differentiation
Volume50
Issue number6
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Transgenic
  • Zebrafish
  • hsp70

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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