Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants

Toru Furukawa; Chiho Sawaguchi; Aiko Watanabe; Maki Takahashi; Mutsumi Nigorikawa; Kayoko Furukawa; Yosuke Iimura; Shinya Kajita; Taichi Oguchi; Yukihiro Ito; Tomonori Sonoki

doi:10.1016/j.jbiosc.2013.05.007

Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants

Toru Furukawa, Chiho Sawaguchi, Aiko Watanabe, Maki Takahashi, Mutsumi Nigorikawa, Kayoko Furukawa, Yosuke Iimura, Shinya Kajita, Taichi Oguchi, Yukihiro Ito, Tomonori Sonoki

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We observed a reduction of lignin content linked to the expression of fungal laccase in rice plants. The lignin content of L-4, which showed the highest LAC activity among transgenic lines produced, was lower than that of the control line. However, this change was not reflected to the saccharification efficiency.

Original language	English
Pages (from-to)	616-619
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	116
Issue number	5
DOIs	https://doi.org/10.1016/j.jbiosc.2013.05.007
Publication status	Published - 2013 Nov

Keywords

Cellulose-binding domain
Fusion protein
Laccase
Oryza sativa L.
Trametes versicolor

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2013.05.007

Cite this

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title = "Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants",

abstract = "We observed a reduction of lignin content linked to the expression of fungal laccase in rice plants. The lignin content of L-4, which showed the highest LAC activity among transgenic lines produced, was lower than that of the control line. However, this change was not reflected to the saccharification efficiency.",

keywords = "Cellulose-binding domain, Fusion protein, Laccase, Oryza sativa L., Trametes versicolor",

author = "Toru Furukawa and Chiho Sawaguchi and Aiko Watanabe and Maki Takahashi and Mutsumi Nigorikawa and Kayoko Furukawa and Yosuke Iimura and Shinya Kajita and Taichi Oguchi and Yukihiro Ito and Tomonori Sonoki",

note = "Funding Information: This work was supported in part by a Hirosaki University Grant for Exploratory Research by Young Scientists (TS), and a Cooperative Research Grant of the Plant Transgenic Design Initiative Program, Gene Research Center, University of Tsukuba (TF, TO, and TS).",

year = "2013",

month = nov,

doi = "10.1016/j.jbiosc.2013.05.007",

language = "English",

volume = "116",

pages = "616--619",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "Elsevier",

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TY - JOUR

T1 - Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants

AU - Furukawa, Toru

AU - Sawaguchi, Chiho

AU - Watanabe, Aiko

AU - Takahashi, Maki

AU - Nigorikawa, Mutsumi

AU - Furukawa, Kayoko

AU - Iimura, Yosuke

AU - Kajita, Shinya

AU - Oguchi, Taichi

AU - Ito, Yukihiro

AU - Sonoki, Tomonori

N1 - Funding Information: This work was supported in part by a Hirosaki University Grant for Exploratory Research by Young Scientists (TS), and a Cooperative Research Grant of the Plant Transgenic Design Initiative Program, Gene Research Center, University of Tsukuba (TF, TO, and TS).

PY - 2013/11

Y1 - 2013/11

N2 - We observed a reduction of lignin content linked to the expression of fungal laccase in rice plants. The lignin content of L-4, which showed the highest LAC activity among transgenic lines produced, was lower than that of the control line. However, this change was not reflected to the saccharification efficiency.

AB - We observed a reduction of lignin content linked to the expression of fungal laccase in rice plants. The lignin content of L-4, which showed the highest LAC activity among transgenic lines produced, was lower than that of the control line. However, this change was not reflected to the saccharification efficiency.

KW - Cellulose-binding domain

KW - Fusion protein

KW - Laccase

KW - Oryza sativa L.

KW - Trametes versicolor

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VL - 116

SP - 616

EP - 619

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -

Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants

Abstract

Keywords

ASJC Scopus subject areas

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