Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

Takakazu Nakabayashi, Hui Ping Wang, Masataka Kinjo, Nobuhiro Ohta

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

Original languageEnglish
Pages (from-to)668-670
Number of pages3
JournalPhotochemical and Photobiological Sciences
Volume7
Issue number6
DOIs
Publication statusPublished - 2008
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Fingerprint Dive into the research topics of 'Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements'. Together they form a unique fingerprint.

  • Cite this