Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell

Takakazu Nakabayashi, Kamlesh Awasthi, Nobuhiro Ohta

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fluorescence lifetime imaging (FLIM) has now been used in many bioscience fields, which comes from the quantification of fluorescence lifetime. The procedure for obtaining lifetime images is very similar to that used in fluorescence microscopy. However, obtaining reliable lifetime images requires an understanding of the theory of fluorescence lifetime, principle of FLIM systems, and evaluation procedure of intracellular environments. In this chapter, the materials, methods, and notes on FLIM measurements have been described, in conjunction with a brief explanation of the background of FLIM.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages121-133
Number of pages13
DOIs
Publication statusPublished - 2017 Jan 1

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1035
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Femtosecond laser
  • Fluorescence lifetime imaging
  • Intracellular environment
  • Intracellular pH
  • Ratiometric method
  • TCSPC

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Nakabayashi, T., Awasthi, K., & Ohta, N. (2017). Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell. In Advances in Experimental Medicine and Biology (pp. 121-133). (Advances in Experimental Medicine and Biology; Vol. 1035). Springer New York LLC. https://doi.org/10.1007/978-3-319-67358-5_8