Application of five‐state structured models for growth and secondary metabolite production in cultures of Nicotiana tabacum

Naomi Shibasaki, Toshikuni Yonemoto, Teiriki Tadaki

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

A new structured model was constructed and applied to batch suspension cultures of Nicotiana tabacum in an airlift bioreactor with a draught tube. This model was developed by modifying the structured model proposed by Bailey and Nicholson1,2 for cultures of Catharanthus roseus cells. In this model, a plant cell was considered to consist of a vacuole and non‐vacuole. A secondary metabolite was also assumed to be synthesized by viable cells and stored at a constant concentration in the vacuole. Parameters for the model equations were estimated based upon experimental data using numerical optimization techniques. The simulated results using these parameters showed that the present model was superior to the earlier model for describing growth and product synthesis during all culture periods for the tobacco cell cultures.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalJournal of Chemical Technology & Biotechnology
Volume58
Issue number2
DOIs
Publication statusPublished - 1993

Keywords

  • Nicotiana tabacum
  • airlift bioreactor
  • product synthesis
  • structured model
  • suspension culture

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry

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