Application of Fiuoresceinisothiocyanate Labeling of Protein to SDS-Electrophoresis and Subsequent Sequence Analysis

Koji Muramoto, Hiroshi Meguro, Katura Tuzimura

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Fiuoresceinisothiocyanate was applied to fluorescent labeling of protein for the determination of molecular weight by SDS polyacrylamide gel electrophoresis. Proteins having molecular weight ranging from 15,000 to 70,000 were tested. The resolved FITC-Iabeled protein was eluted from the gel and was submitted to amino acid sequence analysis.

    Original languageEnglish
    Pages (from-to)2059-2062
    Number of pages4
    JournalAgricultural and Biological Chemistry
    Volume41
    Issue number10
    DOIs
    Publication statusPublished - 1977 Jan 1

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

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