Application of elastic salmon collagen gel to uniaxial stretching culture of human umbilical vein endothelial cells

Toshiyuki Kanayama, Nobuhiro Nagai, Kazuo Mori, Masanobu Munekata

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In this study, we investigated the potential of an elastic salmon collagen gel (e-gel) for use as stretching culture scaffold. First, human umbilical vein endothelial cells (HUVECs) were cultured on the e-gel under static condition, and their growth was evaluated by DNA content measurement, MTT test, and scanning electron microscopy. The results demonstrated steady increases in cell number with culture time. Next, HUVECs were cultured on the e-gel under static condition for 2 d, then uniaxially stretched at a constant frequency (10% elongation at 1 Hz). After the stretching culture for 2 h, the cells oriented perpendicularly to the stretch direction. Moreover, the interleukin-6 and interleukin-8 productions of the cells significantly increased under the stretch condition compared with those under the static condition. These results were in good agreement with the published data in which an elastic silicone membrane was used as a scaffold. In conclusion, the e-gel can be used for stretching culture for vascular tissue engineering.

Original languageEnglish
Pages (from-to)554-557
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume105
Issue number5
DOIs
Publication statusPublished - 2008 May
Externally publishedYes

Keywords

  • elastic collagen
  • endothelial cells
  • salmon collagen
  • scaffold
  • stretching culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Application of elastic salmon collagen gel to uniaxial stretching culture of human umbilical vein endothelial cells'. Together they form a unique fingerprint.

Cite this