Application of combination bubble liposomal amphotericin B and sonication has the dramatic effect on oral candidiasis

M. Yamamoto, K. Iwanaga, T. Okinaga, W. Ariyoshi, K. Tominaga, T. Nishihara

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Objective We previously reported that ultrasound-mediated destruction of microbubbles might be an innovative non-invasive drug delivery system. AmBisome is a single bilayer liposomal drug delivery system consisting of unilamellar bilayer liposomes with amphotericin B, an antifungal drug, intercalated within the membrane. In the present study, we developed a novel fungicidal drug delivery system using ultrasound and bubble liposomal amphotericin B prepared by enclosing perfluoropropane gas within AmBisome. Methods Candida albicans growth inhibition was determined by measuring optical density (OD), counting the number of colony forming units (CFU), and measuring the colony diameters. Result Treatment with bubble liposomal amphotericin B and ultrasound significantly reduced the OD, CFU and diameter of colonies of C. albicans compared with ultrasound alone, liposomal amphotericin B alone, bubble liposomal amphotericin B alone, liposomal amphotericin B with ultrasound. Conclusion These results indicate that treatment with bubble liposomal amphotericin B with ultrasound efficiently inhibited the growth of C. albicans, suggesting this novel approach may have a beneficial effect on the treatment of oral candidal disease.

Original languageEnglish
Pages (from-to)193-197
Number of pages5
JournalJournal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Issue number3
Publication statusPublished - 2017 May
Externally publishedYes


  • Bubble liposome
  • Candida albicans
  • Drug delivery
  • Liposomal amphotericin B
  • Ultrasonication

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Pathology and Forensic Medicine
  • Otorhinolaryngology


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