TY - JOUR
T1 - Application of a gastric cancer cell line (MKN-28) for anti-adenovirus screening using the MTT method
AU - Kodama, Eiichi
AU - Shigeta, Shiro
AU - Suzuki, Toshimitsu
AU - De Clercq, Erik
PY - 1996/7
Y1 - 1996/7
N2 - We established a sensitive and accurate method for screening of anti-adenovirus agents using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. MKN-28 cells, which are well-differentiated stomach adenocarcinoma cells, were used for adenovirus (ADV) infection and examined for the anti-ADV activities of several established anti-herpes virus agents. ADV-11 is the causative agent of respiratory and urinary infections. It frequently causes hemorrhagic cystitis in immunocompromised hosts. One laboratory strain and 4 clinical isolates of ADV-11 were examined, and found susceptible (in order of decreasing activity) to 2-amino-7-[(1,3- dihydroxy-2-propoxy)methyl]purine (S-2242), (S)-9- (3-hydroxy-2-phosphonylmethoxypropyl)adenine[(S)-HPMPA], and (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine[(S)- HPMPC). On the other hand, ganciclovir and iododeoxyuridine were only weakly effective and dextran sulfate was ineffective. Our findings indicate that the MTT assay using MKN-28 cells is applicable to anti-ADV screening. The anti-ADV activity of (S)-HPMPA and (S)-HPMPC was confirmed, and, furthermore, S-2242 emerged as a highly potent and selective inhibitor of ADV-11.
AB - We established a sensitive and accurate method for screening of anti-adenovirus agents using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. MKN-28 cells, which are well-differentiated stomach adenocarcinoma cells, were used for adenovirus (ADV) infection and examined for the anti-ADV activities of several established anti-herpes virus agents. ADV-11 is the causative agent of respiratory and urinary infections. It frequently causes hemorrhagic cystitis in immunocompromised hosts. One laboratory strain and 4 clinical isolates of ADV-11 were examined, and found susceptible (in order of decreasing activity) to 2-amino-7-[(1,3- dihydroxy-2-propoxy)methyl]purine (S-2242), (S)-9- (3-hydroxy-2-phosphonylmethoxypropyl)adenine[(S)-HPMPA], and (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine[(S)- HPMPC). On the other hand, ganciclovir and iododeoxyuridine were only weakly effective and dextran sulfate was ineffective. Our findings indicate that the MTT assay using MKN-28 cells is applicable to anti-ADV screening. The anti-ADV activity of (S)-HPMPA and (S)-HPMPC was confirmed, and, furthermore, S-2242 emerged as a highly potent and selective inhibitor of ADV-11.
KW - Adenovirus
KW - Antiviral screening
KW - MTT method
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U2 - 10.1016/0166-3542(96)06966-5
DO - 10.1016/0166-3542(96)06966-5
M3 - Article
C2 - 8811200
AN - SCOPUS:0030199967
VL - 31
SP - 159
EP - 164
JO - Antiviral Research
JF - Antiviral Research
SN - 0166-3542
IS - 3
ER -