TY - JOUR
T1 - Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes
AU - Igarashi, Aki
AU - Yamagata, Kousuke
AU - Sugai, Tomokazu
AU - Takahashi, Yukari
AU - Sugawara, Emiko
AU - Tamura, Akihiro
AU - Yaegashi, Hajime
AU - Yamagishi, Noriko
AU - Takahashi, Tsubasa
AU - Isogai, Masamichi
AU - Takahashi, Hideki
AU - Yoshikawa, Nobuyuki
N1 - Funding Information:
We thank Prof. N. Kawai, Iwate University for his helpful advice. This work was supported in part by Grant-in-Aids for KAKENHI (no. 19380026, 18380196, 20380025) and the 21st Century of Excellence Program from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2009/4/10
Y1 - 2009/4/10
N2 - Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.
AB - Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.
KW - Apple latent spherical virus vector
KW - Arabidopsis thaliana
KW - Cucurbit
KW - Legume
KW - Tobacco
KW - Virus-induced gene silencing
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U2 - 10.1016/j.virol.2009.01.039
DO - 10.1016/j.virol.2009.01.039
M3 - Article
C2 - 19243807
AN - SCOPUS:62749166968
VL - 386
SP - 407
EP - 416
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -