Apoptotic DNA endonuclease (DNase-γ) gene transfer induces cell death accompanying DNA fragmentation in human glioma cells

Ryuta Saito, Masaaki Mizuno, Toshihiro Kumabe, Takashi Yoshimoto, Sei Ichi Tanuma, Jun Yoshida

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims: Both the genetic restoration of the apoptotic pathway and the introduction of proapoptotic molecules are now drawing attention. Concerning apoptosis of human glioma cells induced by human interferon-β protein, we found that DNA endonuclease (DNase-γ) acts as an executive molecule. The authors investigated whether gene transfer of this DNase-γ exerts some therapeutic effects on human glioma cells. Methods: We transduced U251SP, U251MG, and T98G human glioma cells with DNase-γ gene via multilamellar cationic liposomes, monitored the growth of those cells, and carefully observed the cell-death pattern. Results: DNase-γ gene transfer resulted in an overexpression of DNase-γ protein and induced DNA fragmentation in gene-transferred cells. The cytotoxic effect rose with multiple inoculations of the liposome, suggesting a relationship between its expression and the therapeutic effect. Conclusions: These results demonstrate that DNase-γ gene transfer can induce apoptosis in human glioma cells, indicating its potential to become a future gene therapy strategy.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalJournal of Neuro-Oncology
Volume63
Issue number1
DOIs
Publication statusPublished - 2003 May 1

Keywords

  • Apoptosis
  • DNase-γ
  • Gene transfer
  • Glioma

ASJC Scopus subject areas

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

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